Experimental design
Consulting complete analysis workflow
Primer and probe design → standard reference genes
Assay optimisation and validation
Reference genes validation
Expression profiling // Absolute quantification → more…
Sample preparation and QC
Nucleic acids isolation and purification
Quality control of isolated RNA and DNA:
– isolation yields // concentration – purity // protein contaminants
– integrity // fragment analysis, RIN
DNA or RNA spikes // gDNA ValidPrime // IPC → more…
PCR techniques
End-point PCR // Quantitative PCR 96 // 384
Dye // Probe // Multiplex qPCR
Melting and high resolution melting analysis
Microfluidic chip qPCR // 9216 reactions per run
High throughput gene expression profiling – quantification of 96 genes in 96 samples → more…
Digital PCR
State-of-art technology in PCR, that provide high resolution and sensitivity ideal for:
– Rare mutation detection
– Genotyping // miRNA quantification
– Copy number variation
– Absolute quantification w/o standard → more…
Single cell
Single cell analysis – manual or automated selection and pickup of individual cells → more…
Two-tailed PCR
Specific miRNA quantification using an unique cost effective assay
Dye-based two-tailed primer design
Expression analysis
Validated assay transferred to customer for unlimited use → more…
Automation
Robotic pipetting station for 96 // 384 plates
– high throughput analysis
– ready-to-use plates with mastermixes → more…
NGS RNA-Seq
User-specific experimental design for whole transcriptome analysis
Library preparation and quality control
Single cell RNA-Seq
Basic data analysis → more…
Data analysis
Data quality // Differential expression analysis
Selection of reference genes // Normalization
Descriptive statistic // non-parametric tests
Multivariate analysis (PCA) // Genex
Outputs for Materials & Methods section → more…