PCR
Bio-Rad // CFX96
PCR cycler for 96 well plates with real-time reading of fluerescence. 5 channels, melting analysis.
Bio-Rad // CFX384
PCR cycler for 384 well plates with real-time reading of fluerescence. 5 channels, melting analysis.
Roche // LightCycler 480 II
PCR cycler for 96 or 384 well plates /interchangable platform with real-time reading of fluerescence. 5 channels, melting analysis.
Our instruments utilize detections systems for variety of dyes, inluding mostly used real-time PCR systems (SYBR green I, FAM, HEX, Cy5). Real-time PCR instruments can be used even for aplications based on FRET (protein-protein, DNA-protein interactions etc.) Contact us for detail technical specifications for your experimental requirements.
Bio-Rad // C1000
PCR cycler for 96 well format without optical unit, for end-point PCR or RT reactions etc.
Digital PCR
Bio-Rad // QX200
System for digital PCR based on emulsion particles. Easy to use, high quality results, reasonable cost per sample. Manifold for particles forming for 8 samples, PCR and read-out of results in standard 96 well plate.
High throughput qPCR
Fluidigm // Biomark and NanoFlex
Microfluidic chip qPCR system allows up to 9216 reactions per run. Two chip platforms, one for 48 samples with 48 assays or 96 samples with 96 assays respectively. Complet experiment including loading and reading chip take circa 3 hours.
DNA/RNA quality control
Advanced Analytical // Fragment analyzer
Powerful but cost-effective tool for complex analysis of nucleic acids purity and integrity. Based on capillary electrophoresis. 12 channels system for 96 well format.
Thermo Scientific // NanoDrop 8000
Easy-to-use spectrophotometer for analysis of DNA/RNA/protein samples. One or 8 channels in once, minimum volume of sample 1 μl.
Invitrogen // Qubit
Dye-based photometer for sensitive quantification of DNA, RNA and proteins. Ideal for precise normalization based on RNA amount. Limits of detection in pg/μl scales (depends on target and used dye kit).
Single cell analysis
ALS // AVISO CellCelector
Fully automated microscopic micromanipulator available for pick-up of tagged cells in suspension. Each selected cell is transfered to individual well in 96 wells plate. System is dedicated for single cell analyses, e.g. expression profiling.
Automation
Eppendorf // epMotion P5073
Robotic pipetting station for 96 and 384 well plates. Ideal for high throughput analyses or preparing ready-to-use plates with PCR mastermixes.
Software
MultiD Analyses // GenEx
complex software tool for processing real-time PCR data (relative quantification, reference gene validation etc.).
Premier Biosoft // AlleleID and Beacon Designer
Tolls for design and validation of primers and probes for PCR assays.
Bio-Rad //Precision Met Analysis
Tool for evaluate High Resolution Melting analysis performed on Bio-Rad PCR instruments. Useable for SNP genotyping.
- All instruments in GeneCore facility can be used by our clients and partners upon appropriate training. You may use your own disposable materials and reagents, or they can be provided by GeneCore. Contact us for prices and detail options.